Ukrainian Journal of Physical Optics


2024 Volume 25, Issue 4


ISSN 1816-2002 (Online), ISSN 1609-1833 (Print)

MECHANISMS AND PARAMETERS OF THE BINDING OF THE FLAVONOID QUERCETIN TO DNA IN AN AQUEOUS SOLUTION

S. Kutovyy, D. Fedorovich, R. Savchuk, A. Naumenko and L. Palchykivska

Author Information

S. Kutovyy ORCID logoTaras Shevchenko National University of Kyiv, Faculty of Physics;
D. Fedorovich, Taras Shevchenko National University of Kyiv, Faculty of Physics;
R. Savchuk, Taras Shevchenko National University of Kyiv, Faculty of Physics;
A. Naumenko ORCID logoTaras Shevchenko National University of Kyiv, Faculty of Physics;
L. Palchykivska ORCID logoInstitute of Molecular Biology and Genetics (NAS of Ukraine), Department of Molecular and Quantum Biophysics


ABSTRACT

The interaction of the flavonoid quercetin with the DNA macromolecule in an aqueous solution has been studied through optical spectroscopy - electron absorption and fluorescence. Based on the obtained results, the dependence of the spectral characteristics on the concentration ratio N/c between the DNA base pairs and the ligand molecules was constructed. Using the system of modified equations of Scatchard and McGhee-von Hippel, the binding parameters of quercetin with DNA are determined. The binding parameters to the DNA of other molecules we studied earlier are given for comparison.

Keywords: DNA, quercetin, McGhee-von Hippel binding equation, absorption spectra, fluorescence spectra.

UDC: 535.34; 535.37

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    Взаємодію флавоноїду кверцетину з макромолекулою ДНК у водному розчині досліджено методами оптичної спектроскопії – електронного поглинання та флуоресценції. На основі отриманих результатів побудовано залежності спектральних характеристик від відношення концентрацій N/c між парами основ ДНК і молекулами ліганду. За допомогою системи модифікованих рівнянь Скетчарда і МакГі-фон Гіппеля визначено параметри зв'язування кверцетину з ДНК. Користуючись системою модифікованих рівнянь Скетчарда та МакГі-фон Хіппеля, визначено параметри зв'язування кверцетину з ДНК. Для порівняння наведено параметри зв'язування з ДНК інших досліджених нами раніше молекул. Ключові слова: ДНК, кверцетин, рівняння зв’язування МакГі - фон Хіппеля, спектри поглинання, спектри флуоресценції.

    Ключові слова: ДНК, кверцетин, рівняння зв’язування МакГі - фон Хіппеля, спектри поглинання, спектри флуоресценції.

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