Ukrainian Journal of Physical Optics
2024 Volume 25, Issue 4
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MECHANISMS AND PARAMETERS OF THE BINDING OF THE FLAVONOID QUERCETIN TO DNA IN AN AQUEOUS SOLUTION
S. Kutovyy, D. Fedorovich, R. Savchuk, A. Naumenko and L. Palchykivska
S. Kutovyy Taras Shevchenko National University of Kyiv, Faculty of Physics;
D. Fedorovich, Taras Shevchenko National University of Kyiv, Faculty of Physics;
R. Savchuk, Taras Shevchenko National University of Kyiv, Faculty of Physics;
A. Naumenko
Taras Shevchenko National University of Kyiv, Faculty of Physics;
L. Palchykivska
Institute of Molecular Biology and Genetics (NAS of Ukraine), Department of Molecular and Quantum Biophysics
Ukr. J. Phys. Opt.
Vol. 25
,
Issue 4 , pp. 04008 - 04021 (2024).
doi:10.3116/16091833/Ukr.J.Phys.Opt.2024.04008
ABSTRACT
Keywords:
DNA, quercetin, McGhee-von Hippel binding equation, absorption spectra, fluorescence spectra.
UDC:
535.34; 535.37
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Взаємодію флавоноїду кверцетину з макромолекулою ДНК у водному розчині досліджено методами оптичної спектроскопії – електронного поглинання та флуоресценції. На основі отриманих результатів побудовано залежності спектральних характеристик від відношення концентрацій N/c між парами основ ДНК і молекулами ліганду. За допомогою системи модифікованих рівнянь Скетчарда і МакГі-фон Гіппеля визначено параметри зв'язування кверцетину з ДНК. Користуючись системою модифікованих рівнянь Скетчарда та МакГі-фон Хіппеля, визначено параметри зв'язування кверцетину з ДНК. Для порівняння наведено параметри зв'язування з ДНК інших досліджених нами раніше молекул.
Ключові слова: ДНК, кверцетин, рівняння зв’язування МакГі - фон Хіппеля, спектри поглинання, спектри флуоресценції.
Ключові слова: ДНК, кверцетин, рівняння зв’язування МакГі - фон Хіппеля, спектри поглинання, спектри флуоресценції.
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